In Iran, a large body of research has demonstrated the importance of the antagonist fungus, Talaromycesflavus, in inhibiting the growth of some important soil-borne pathogensuch as Rhizoctoniasolani, Verticilliumdahlia, and Fusariumoxysporum. According to results obtained up to this stage of studies, commercialization of this fungus bioformulationis of particular importance. Since marketing is considered to be an important factor for continued commercialization, paying attention to bioformulationtype with eveneasier application, low consumption, and an appearance similar to common chemical fungicides can be largely influential in attracting relevant consumers and having a successful marketing. To continue the above research, preparations were made from different bioformulations of the aforesaid biological agent with an emphasis on facilitating the application and possibly with similar formulations of chemical fungicides available in the market to investigate their efficacy in biological control of plant disease. Eight liquid bioformulations was prepared from T. flavus using a fermenter by manipulation of several important factors, namely two different temperatures, two types of media, and two types of stabilizers. Among the eight prepared liquid bioformulations, the most effective ones were selected for greenhouse evaluation in terms of the lowest infection percentage, maximum sporulation and active population, and the highest mycelial mass. These bioformulations were CDB + NaNO3 + 30 °C, CDB + NaNO3 + 25 °C, CDB + D-cycloserine+ 30 °C, and PDA + D-cycloserine+ 25 °C. According to results of greenhouse study on four formulations with two different application methods (addition to soil and seed impregnation), two treatments of CDB + NaNO3 + 25 °C and PDA + D-cycloserine+ 25 °C with seed impregnation method were the most effective treatments in increasing the number of healthy sugar beet plants.