The research work involves developing a better and more reliable method for the bio-industrialization of zinc oxide nanoparticles through the green method. Nanotechnology is expected to open up new ways of disease control and prevention by using large-scale tailoring of atomic materials. Prominent nanoparticles with antimicrobial properties include metallic nanoparticles, which exhibit increased chemical activity due to their surface ratios to their large size and crystal surface structure. The study of microbial nanoparticles comes in a timely manner especially given the recent increase in new resistant bacterial strains of the most effective antibiotic. The current work studies the effect of silver nanoparticles in the range of 1 to 100 nm on Gram negative bacteria using the HAADF, which scans the electron microscopy of transmission (STEM). This study identifies the antimicrobial effectiveness of ZnO particles synthesized against the clinical and normative strains of S. aureus, P. aeruginosa and the standard strain of E. coli.