Adhesions type1 fimbriae, P- fimbriae, S-fimbriae, and A- fimbriae adhesion and fimbria is assembled by chaperone-usher pathway. In the currentre search, 100 Uropatho-genic E. coli isolates was genotypic ally characterized-by polymerase-chain reactions (PCR) assay. The development of PCR assay for fimbria related to genes D-film, C-film, pap CVD, SAF D/E, SFA F, and a B/C. The results revealed that the percentage of the genes were as follow: D-film(100%),C-film(100%), PAP ClD(54%),SFAD/E (18%), SFA F (18%) and AFA B/C (5%). The results demonstrated that chaperone-usher of type 1 fimbriae was predominate, which might contribute to E.coli virulence. Current work, demonstrate the adhesions to be multiples with a-single isolate and that various virulence genes-can occur in the associations. The expression of adhesion factor was determined as followed (92%, 62%) and results showed a close relationship between phenotypic and genotypic results for type 1 fimbriae and CAFl also between P fimbriae and CAFШ.